Ogami is hired to kill a tattooed female assassin. Gunbei Yagyu, an enemy samurai, happens upon Ogami's son, and sees his chance for revenge.
Five warriors challenge Ogami to duels. Each holds a part of information needed to complete the assassination of a mad clan leader.
In the sixth and final film of the Lone Wolf and Cub series, the final conflict between Ogami Itto and the Yagyu clan is carried out.